Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33144 | 5' | -57.6 | NC_007497.1 | + | 43275 | 0.66 | 0.468928 |
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Target: 5'- cUCCGGCAacccugcauugaUCCAgaaguCUGCGCCGGc-- -3' miRNA: 3'- aAGGUCGU------------AGGUau---GGCGCGGCCucu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 36981 | 0.68 | 0.37338 |
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Target: 5'- -aUCAGCccgCCG-GCgGCGCCGGAGGc -3' miRNA: 3'- aaGGUCGua-GGUaUGgCGCGGCCUCU- -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 23504 | 0.68 | 0.370724 |
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Target: 5'- -gCCGGCGcCCGccGCCGCGCCcagcagcgcgaccaGGAGAu -3' miRNA: 3'- aaGGUCGUaGGUa-UGGCGCGG--------------CCUCU- -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 23382 | 0.68 | 0.359364 |
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Target: 5'- uUUCCaucgucugaaagcagGGCGUCCGUGaCGCGCUGGAu- -3' miRNA: 3'- -AAGG---------------UCGUAGGUAUgGCGCGGCCUcu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 38161 | 0.69 | 0.347404 |
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Target: 5'- -gCCGGCGaCCGUGgCGCGCCuGAGc -3' miRNA: 3'- aaGGUCGUaGGUAUgGCGCGGcCUCu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 42390 | 0.69 | 0.314822 |
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Target: 5'- -gCCAcCAUUCAgcucGCCGCGCCGGAu- -3' miRNA: 3'- aaGGUcGUAGGUa---UGGCGCGGCCUcu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 33107 | 0.7 | 0.277416 |
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Target: 5'- -cCCGGCuacUCCcgGCCGUGCCGGu-- -3' miRNA: 3'- aaGGUCGu--AGGuaUGGCGCGGCCucu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 31226 | 0.71 | 0.263477 |
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Target: 5'- gUCCAacGCAUCCuUGCUGCGCUGGu-- -3' miRNA: 3'- aAGGU--CGUAGGuAUGGCGCGGCCucu -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 2894 | 0.76 | 0.112665 |
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Target: 5'- gUCCGcCGUCCAca-CGCGCCGGAGAu -3' miRNA: 3'- aAGGUcGUAGGUaugGCGCGGCCUCU- -5' |
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| 33144 | 5' | -57.6 | NC_007497.1 | + | 26925 | 0.94 | 0.005419 |
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Target: 5'- uUUCCAGCAUCCAUACCGCGCC-GAGAc -3' miRNA: 3'- -AAGGUCGUAGGUAUGGCGCGGcCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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