Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33145 | 5' | -57.1 | NC_007497.1 | + | 24519 | 0.66 | 0.574855 |
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Target: 5'- gCGuACGcGCGGGAUUGCCacgGcGGCGAa -3' miRNA: 3'- -GC-UGC-CGUCCUAGCGGca-CuUCGCUa -5' |
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| 33145 | 5' | -57.1 | NC_007497.1 | + | 11865 | 0.67 | 0.499919 |
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Target: 5'- aGACGGCAcguuGGGcUCGCCG-GAauaucuGGCGGa -3' miRNA: 3'- gCUGCCGU----CCU-AGCGGCaCU------UCGCUa -5' |
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| 33145 | 5' | -57.1 | NC_007497.1 | + | 18636 | 0.7 | 0.340092 |
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Target: 5'- cCGGCGuGCAGGGgcaGCCGacuaccgaUGAGGCGGc -3' miRNA: 3'- -GCUGC-CGUCCUag-CGGC--------ACUUCGCUa -5' |
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| 33145 | 5' | -57.1 | NC_007497.1 | + | 20687 | 0.73 | 0.226571 |
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Target: 5'- uGGCGGaAGGAUCGCCGcGAAcagcGCGAg -3' miRNA: 3'- gCUGCCgUCCUAGCGGCaCUU----CGCUa -5' |
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| 33145 | 5' | -57.1 | NC_007497.1 | + | 2652 | 0.77 | 0.107818 |
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Target: 5'- uCGAUGGCGcGGAUCG-UGUGGAGCGGUa -3' miRNA: 3'- -GCUGCCGU-CCUAGCgGCACUUCGCUA- -5' |
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| 33145 | 5' | -57.1 | NC_007497.1 | + | 27029 | 1.05 | 0.000928 |
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Target: 5'- cCGACGGCAGGAUCGCCGUGAAGCGAUc -3' miRNA: 3'- -GCUGCCGUCCUAGCGGCACUUCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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