miRNA: 3'- caAGUGGCAC---AG-CCGC-------------UAGUCGCUCg -5'
miRNA: 3'- caAGUGGCACagcCGCUAGU------------CGCUCG- -5'
miRNA: 3'- caagUGGCac--AGCCGCUa--GUCGCUCG- -5'
miRNA: 3'- caagUGGC-ACAGCCGCUAGUCGCUCG- -5'
miRNA: 3'- -CAAGUGGCACAGCCGCUAGUCGCUCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33149 | 5' | -54.5 | NC_007497.1 | + | 3594 | 0.7 | 0.468748 |
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Target: 5'- -aUCACCGUGaaaUCgGGCGuguagaacguguuGUCGGCGAGa -3' miRNA: 3'- caAGUGGCAC---AG-CCGC-------------UAGUCGCUCg -5' |
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| 33149 | 5' | -54.5 | NC_007497.1 | + | 27040 | 0.7 | 0.459598 |
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Target: 5'- -aUCGCCGUGaa-GCGAUCAcccggcgccacuGCGGGCg -3' miRNA: 3'- caAGUGGCACagcCGCUAGU------------CGCUCG- -5' |
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| 33149 | 5' | -54.5 | NC_007497.1 | + | 671 | 0.73 | 0.331057 |
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Target: 5'- ---aGCCGaccuUCGGCGAaugCAGCGAGCa -3' miRNA: 3'- caagUGGCac--AGCCGCUa--GUCGCUCG- -5' |
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| 33149 | 5' | -54.5 | NC_007497.1 | + | 10263 | 0.81 | 0.091793 |
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Target: 5'- ---gGCUGuUGUCGGUGGUCGGCGGGCu -3' miRNA: 3'- caagUGGC-ACAGCCGCUAGUCGCUCG- -5' |
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| 33149 | 5' | -54.5 | NC_007497.1 | + | 27988 | 1.11 | 0.000677 |
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Target: 5'- uGUUCACCGUGUCGGCGAUCAGCGAGCu -3' miRNA: 3'- -CAAGUGGCACAGCCGCUAGUCGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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