Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
33155 | 5' | -58.5 | NC_007497.1 | + | 6241 | 0.66 | 0.444032 |
Target: 5'- uCGCAGCAUcgCGCCcgacauGCCGAgccguggCGCc- -3' miRNA: 3'- uGCGUCGUGuaGCGG------CGGCUa------GCGac -5' |
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33155 | 5' | -58.5 | NC_007497.1 | + | 36448 | 0.66 | 0.443056 |
Target: 5'- cGCGCAGCucggcaaGCGUC-CUGCCGAgcCGCa- -3' miRNA: 3'- -UGCGUCG-------UGUAGcGGCGGCUa-GCGac -5' |
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33155 | 5' | -58.5 | NC_007497.1 | + | 18283 | 0.66 | 0.485009 |
Target: 5'- cGCGCAGaacgugaugaacgugACGUCGCUGCCGGcCGUc- -3' miRNA: 3'- -UGCGUCg--------------UGUAGCGGCGGCUaGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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