Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33155 | 5' | -58.5 | NC_007497.1 | + | 42329 | 0.75 | 0.126847 |
Target: 5'- uGCGCgugauGGCGCGUCGCCGCgCGAaCGCc- -3' miRNA: 3'- -UGCG-----UCGUGUAGCGGCG-GCUaGCGac -5' |
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33155 | 5' | -58.5 | NC_007497.1 | + | 36168 | 0.77 | 0.093012 |
Target: 5'- gGCGgGGCGCGUCgGCCGcCCGGUCGUg- -3' miRNA: 3'- -UGCgUCGUGUAG-CGGC-GGCUAGCGac -5' |
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33155 | 5' | -58.5 | NC_007497.1 | + | 30573 | 1.07 | 0.000477 |
Target: 5'- aACGCAGCACAUCGCCGCCGAUCGCUGu -3' miRNA: 3'- -UGCGUCGUGUAGCGGCGGCUAGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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