Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33160 | 5' | -51.7 | NC_007497.1 | + | 43014 | 0.66 | 0.85494 |
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Target: 5'- cGCggucGUCGAGGUGGauGCGUUCAUc- -3' miRNA: 3'- -CGac--UAGCUCUAUCggCGCAAGUGca -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 35949 | 0.66 | 0.846126 |
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Target: 5'- aGCUGAUgGAugccGAacGCCGCGgcCGCGg -3' miRNA: 3'- -CGACUAgCU----CUauCGGCGCaaGUGCa -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 40520 | 0.67 | 0.808555 |
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Target: 5'- uGCUGAUCGGcGAcgcacacGCCGCucaUCGCGUg -3' miRNA: 3'- -CGACUAGCU-CUau-----CGGCGca-AGUGCA- -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 17500 | 0.67 | 0.778251 |
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Target: 5'- -aUGAUCGAGAc-GCCGCccgCGCGg -3' miRNA: 3'- cgACUAGCUCUauCGGCGcaaGUGCa -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 36330 | 0.68 | 0.735627 |
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Target: 5'- gGCUGAUCGuc--GGCUGCGgaacuccagugUCGCGUg -3' miRNA: 3'- -CGACUAGCucuaUCGGCGCa----------AGUGCA- -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 2705 | 0.68 | 0.724659 |
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Target: 5'- --gGAUCGAGGUucggcaaaaGGCCGCc-UCACGUc -3' miRNA: 3'- cgaCUAGCUCUA---------UCGGCGcaAGUGCA- -5' |
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| 33160 | 5' | -51.7 | NC_007497.1 | + | 32848 | 1.1 | 0.001684 |
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Target: 5'- cGCUGAUCGAGAUAGCCGCGUUCACGUu -3' miRNA: 3'- -CGACUAGCUCUAUCGGCGCAAGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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