Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33161 | 5' | -49.7 | NC_007497.1 | + | 32753 | 0.72 | 0.651978 |
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Target: 5'- -cUUCCUCGGCGCgcccagCGGcGUUcUCGGCg -3' miRNA: 3'- aaAAGGAGCUGCG------GCUcUAAaAGCCG- -5' |
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| 33161 | 5' | -49.7 | NC_007497.1 | + | 10426 | 0.72 | 0.617086 |
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Target: 5'- -cUUCaguUCGGCGCCGAagccGUUUUUGGCa -3' miRNA: 3'- aaAAGg--AGCUGCGGCUc---UAAAAGCCG- -5' |
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| 33161 | 5' | -49.7 | NC_007497.1 | + | 1456 | 0.72 | 0.605469 |
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Target: 5'- ---aCCUCaACGUCGAGA---UCGGCa -3' miRNA: 3'- aaaaGGAGcUGCGGCUCUaaaAGCCG- -5' |
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| 33161 | 5' | -49.7 | NC_007497.1 | + | 5454 | 0.74 | 0.492307 |
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Target: 5'- ----aCUCGACGCCGAuucucUUCGGCg -3' miRNA: 3'- aaaagGAGCUGCGGCUcuaa-AAGCCG- -5' |
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| 33161 | 5' | -49.7 | NC_007497.1 | + | 5960 | 0.76 | 0.419143 |
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Target: 5'- -gUUgCUCGACGCCGcGAcacagUCGGCg -3' miRNA: 3'- aaAAgGAGCUGCGGCuCUaaa--AGCCG- -5' |
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| 33161 | 5' | -49.7 | NC_007497.1 | + | 34144 | 1.09 | 0.002846 |
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Target: 5'- cUUUUCCUCGACGCCGAGAUUUUCGGCc -3' miRNA: 3'- -AAAAGGAGCUGCGGCUCUAAAAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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