Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33165 | 3' | -58.6 | NC_007497.1 | + | 35469 | 0.71 | 0.244148 |
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Target: 5'- cGCGUCGGUacuaGCGCCG-GCA-UUCCg- -3' miRNA: 3'- -CGCAGCCGg---UGCGGCcCGUuAAGGag -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 12344 | 0.71 | 0.231859 |
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Target: 5'- uGCGaccggCGGCCACgucacgggGCCGGGCAcgUCgaCg -3' miRNA: 3'- -CGCa----GCCGGUG--------CGGCCCGUuaAGgaG- -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 9945 | 0.72 | 0.220096 |
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Target: 5'- cGCGagaccgCGGCCGCGCaGGGCGAg--CUCg -3' miRNA: 3'- -CGCa-----GCCGGUGCGgCCCGUUaagGAG- -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 43814 | 0.74 | 0.139389 |
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Target: 5'- uGCGUCGGCCGCGUacugcuCGGGCuucagCCa- -3' miRNA: 3'- -CGCAGCCGGUGCG------GCCCGuuaa-GGag -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 4198 | 0.75 | 0.128316 |
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Target: 5'- aGCGUCgauGGCgCGCGCCGGGU-AUUCC-Cg -3' miRNA: 3'- -CGCAG---CCG-GUGCGGCCCGuUAAGGaG- -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 9158 | 0.76 | 0.114816 |
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Target: 5'- cGCGUCgGGCCAguUGCgCGGGCAGgaCCUUa -3' miRNA: 3'- -CGCAG-CCGGU--GCG-GCCCGUUaaGGAG- -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 15416 | 0.79 | 0.066477 |
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Target: 5'- aCGUCGGCCGCGacguggcgcucgaaCCGGGCGAcgUCCUg -3' miRNA: 3'- cGCAGCCGGUGC--------------GGCCCGUUa-AGGAg -5' |
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| 33165 | 3' | -58.6 | NC_007497.1 | + | 35586 | 1.1 | 0.000295 |
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Target: 5'- cGCGUCGGCCACGCCGGGCAAUUCCUCc -3' miRNA: 3'- -CGCAGCCGGUGCGGCCCGUUAAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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