Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33169 | 3' | -54.3 | NC_007497.1 | + | 38104 | 0.67 | 0.648938 |
Target: 5'- -cGGGUAUCGCaacCGCCGGuguccguGUUCGg -3' miRNA: 3'- aaCCCGUAGCGacaGCGGCU-------UAAGCg -5' |
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33169 | 3' | -54.3 | NC_007497.1 | + | 39187 | 0.72 | 0.332029 |
Target: 5'- -gGGGCGUCGUUGaCGCCccuUUCGUu -3' miRNA: 3'- aaCCCGUAGCGACaGCGGcuuAAGCG- -5' |
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33169 | 3' | -54.3 | NC_007497.1 | + | 41527 | 0.69 | 0.483685 |
Target: 5'- -aGGGCG-CGCUGUUGCgCGGca-CGCu -3' miRNA: 3'- aaCCCGUaGCGACAGCG-GCUuaaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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