Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33169 | 5' | -55.4 | NC_007497.1 | + | 5868 | 0.69 | 0.506587 |
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Target: 5'- cGGGCGGaCUCG-CGgagUGGUugGCGAg -3' miRNA: 3'- -CCCGCCaGAGCuGUau-GUCGugCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 41236 | 0.69 | 0.506587 |
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Target: 5'- cGGGCGG-CgaacgcCGACgAUGCGGCG-GCGAu -3' miRNA: 3'- -CCCGCCaGa-----GCUG-UAUGUCGUgCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 34858 | 0.7 | 0.459381 |
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Target: 5'- -cGCGGUCUCGcgcgcgaucacggccGCAUACGGCGucccgGCGAu -3' miRNA: 3'- ccCGCCAGAGC---------------UGUAUGUCGUg----CGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 22606 | 0.7 | 0.455386 |
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Target: 5'- uGGCGGaucaguUCUauaagGACAUucgcaucuuuGCAGCGCGCGAg -3' miRNA: 3'- cCCGCC------AGAg----CUGUA----------UGUCGUGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 1077 | 0.71 | 0.397844 |
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Target: 5'- cGGCaGGUCaCGAgGgGCAGCGCGCGc -3' miRNA: 3'- cCCG-CCAGaGCUgUaUGUCGUGCGCu -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 20379 | 0.73 | 0.283062 |
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Target: 5'- aGGGgGcaCUCGGCGUAUuccuGCGCGCGAu -3' miRNA: 3'- -CCCgCcaGAGCUGUAUGu---CGUGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 37796 | 0.74 | 0.268983 |
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Target: 5'- -cGUGGUgUCGcGCGUgaGCGGCACGCGAg -3' miRNA: 3'- ccCGCCAgAGC-UGUA--UGUCGUGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 32375 | 0.74 | 0.236259 |
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Target: 5'- cGGCGGUCgCGACGU-CGGUuuCGCGAa -3' miRNA: 3'- cCCGCCAGaGCUGUAuGUCGu-GCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 36662 | 1.11 | 0.000597 |
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Target: 5'- uGGGCGGUCUCGACAUACAGCACGCGAa -3' miRNA: 3'- -CCCGCCAGAGCUGUAUGUCGUGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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