Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33170 | 5' | -57.5 | NC_007497.1 | + | 38533 | 0.69 | 0.384365 |
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Target: 5'- gCuGGCGCCAcggcucggcaUGUCgggCGCGaUGCUGCg -3' miRNA: 3'- gGuUCGCGGU----------ACAGa--GCGC-ACGGCGa -5' |
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| 33170 | 5' | -57.5 | NC_007497.1 | + | 38598 | 0.69 | 0.349717 |
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Target: 5'- cCCAcaaGCUGaGUCUCGCGgagGCCGCUc -3' miRNA: 3'- -GGUucgCGGUaCAGAGCGCa--CGGCGA- -5' |
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| 33170 | 5' | -57.5 | NC_007497.1 | + | 40620 | 0.68 | 0.411802 |
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Target: 5'- -uGAGCGCCAuUGcCUUGCG-GCCGg- -3' miRNA: 3'- ggUUCGCGGU-ACaGAGCGCaCGGCga -5' |
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| 33170 | 5' | -57.5 | NC_007497.1 | + | 41422 | 0.73 | 0.198061 |
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Target: 5'- aCCGAGCGCCuucgagaagaaagcGgcuugGUCguucaGCGUGCCGCg -3' miRNA: 3'- -GGUUCGCGG--------------Ua----CAGag---CGCACGGCGa -5' |
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| 33170 | 5' | -57.5 | NC_007497.1 | + | 43991 | 0.66 | 0.500836 |
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Target: 5'- aCGGGCGCC--GUCggCGUGaucgaUGCCGCa -3' miRNA: 3'- gGUUCGCGGuaCAGa-GCGC-----ACGGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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