Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33170 | 5' | -57.5 | NC_007497.1 | + | 15395 | 0.71 | 0.287292 |
Target: 5'- aCCAGGaCGCCGguuuUCUCGaCGUcgGCCGCg -3' miRNA: 3'- -GGUUC-GCGGUac--AGAGC-GCA--CGGCGa -5' |
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33170 | 5' | -57.5 | NC_007497.1 | + | 11725 | 0.71 | 0.259519 |
Target: 5'- uCCGAGCGUCAgcgagagGUCgCGCGagagcugGCCGCg -3' miRNA: 3'- -GGUUCGCGGUa------CAGaGCGCa------CGGCGa -5' |
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33170 | 5' | -57.5 | NC_007497.1 | + | 41422 | 0.73 | 0.198061 |
Target: 5'- aCCGAGCGCCuucgagaagaaagcGgcuugGUCguucaGCGUGCCGCg -3' miRNA: 3'- -GGUUCGCGG--------------Ua----CAGag---CGCACGGCGa -5' |
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33170 | 5' | -57.5 | NC_007497.1 | + | 4569 | 0.75 | 0.144006 |
Target: 5'- aCGAGCGCCGcGUCUUGCGUcgauCCGCc -3' miRNA: 3'- gGUUCGCGGUaCAGAGCGCAc---GGCGa -5' |
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33170 | 5' | -57.5 | NC_007497.1 | + | 37710 | 1.09 | 0.000495 |
Target: 5'- gCCAAGCGCCAUGUCUCGCGUGCCGCUc -3' miRNA: 3'- -GGUUCGCGGUACAGAGCGCACGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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