Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33174 | 3' | -56.3 | NC_007497.1 | + | 16731 | 0.66 | 0.612621 |
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Target: 5'- gCGAGcgGCgCGcuCCCGgcguaGCGCAUGCGc -3' miRNA: 3'- -GCUCuaCG-GCc-GGGCaa---CGCGUAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 1912 | 0.66 | 0.590436 |
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Target: 5'- ---uGUGCCGGCCCGcagGCaGC-UGCAc -3' miRNA: 3'- gcucUACGGCCGGGCaa-CG-CGuAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 5578 | 0.66 | 0.557448 |
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Target: 5'- --uGAUGCCGcGCCCaugucGUUGCGUcuaagcucAUACAg -3' miRNA: 3'- gcuCUACGGC-CGGG-----CAACGCG--------UAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 14920 | 0.67 | 0.503779 |
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Target: 5'- uCGAGAUuUCGGCCaUGUUGgGCgAUGCGg -3' miRNA: 3'- -GCUCUAcGGCCGG-GCAACgCG-UAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 28116 | 0.68 | 0.480872 |
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Target: 5'- cCGAGugcgaaauccgGCUGGCUgG-UGCGCGUGCGg -3' miRNA: 3'- -GCUCua---------CGGCCGGgCaACGCGUAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 18502 | 0.69 | 0.423093 |
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Target: 5'- gCGAGAgaacugGCCGGCCCGacgauuCGCGaGCGg -3' miRNA: 3'- -GCUCUa-----CGGCCGGGCaac---GCGUaUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 29202 | 0.72 | 0.259421 |
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Target: 5'- aCGGGAUcGCUGGCaCgCGUUucggGCGCGUGCAu -3' miRNA: 3'- -GCUCUA-CGGCCG-G-GCAA----CGCGUAUGU- -5' |
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| 33174 | 3' | -56.3 | NC_007497.1 | + | 40767 | 1.09 | 0.000589 |
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Target: 5'- cCGAGAUGCCGGCCCGUUGCGCAUACAg -3' miRNA: 3'- -GCUCUACGGCCGGGCAACGCGUAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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