Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33175 | 5' | -57.1 | NC_007497.1 | + | 27588 | 0.69 | 0.403716 |
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Target: 5'- gCAUCGCcuacuGggGGCCGGGaGCUGUCGuCg -3' miRNA: 3'- gGUAGCG-----CuaCUGGCUC-CGACGGCuG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 3783 | 0.69 | 0.3856 |
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Target: 5'- gCCggCGCGAUgaacaaGACCGAGcagcGCUacGCCGACc -3' miRNA: 3'- -GGuaGCGCUA------CUGGCUC----CGA--CGGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 1197 | 0.69 | 0.376745 |
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Target: 5'- uCCG-CGCGAagGugUGGGaGCUGCCGGa -3' miRNA: 3'- -GGUaGCGCUa-CugGCUC-CGACGGCUg -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 22750 | 0.7 | 0.350179 |
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Target: 5'- aCAUCGUGAUGAgCGAgaauaauGGCguagGcCCGACu -3' miRNA: 3'- gGUAGCGCUACUgGCU-------CCGa---C-GGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 28835 | 0.7 | 0.34272 |
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Target: 5'- cCCAUCGCGAUcagcGACgGuuGCUcaaacacgaaGCCGACg -3' miRNA: 3'- -GGUAGCGCUA----CUGgCucCGA----------CGGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 40984 | 0.72 | 0.250405 |
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Target: 5'- gUCAUCGCGAUGGCCGGcgacgagauccugauGGgUGaCGGCg -3' miRNA: 3'- -GGUAGCGCUACUGGCU---------------CCgACgGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 35141 | 0.73 | 0.235336 |
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Target: 5'- -gGUCGCGGcgcaGGCCGAGcUUGCCGGCa -3' miRNA: 3'- ggUAGCGCUa---CUGGCUCcGACGGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 36673 | 0.75 | 0.171065 |
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Target: 5'- cCCAa-GCGGUcGCCGAGGCguacggGCCGGCa -3' miRNA: 3'- -GGUagCGCUAcUGGCUCCGa-----CGGCUG- -5' |
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| 33175 | 5' | -57.1 | NC_007497.1 | + | 40927 | 1.12 | 0.000358 |
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Target: 5'- gCCAUCGCGAUGACCGAGGCUGCCGACg -3' miRNA: 3'- -GGUAGCGCUACUGGCUCCGACGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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