Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33176 | 5' | -58.4 | NC_007497.1 | + | 41355 | 0.67 | 0.409434 |
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Target: 5'- -aCAGAAGGGGA-GUGGCGaugaGCCu-- -3' miRNA: 3'- uaGUCUUCCCCUgCGCCGUg---UGGcau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 36947 | 0.68 | 0.355577 |
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Target: 5'- -gCuGgcGGGGugGCGGCACgaauucauGCCGa- -3' miRNA: 3'- uaGuCuuCCCCugCGCCGUG--------UGGCau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 34085 | 0.68 | 0.330581 |
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Target: 5'- -gCAGggGccgcggccgacaGGGGCGCGcGCACGCUGg- -3' miRNA: 3'- uaGUCuuC------------CCCUGCGC-CGUGUGGCau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 34966 | 0.68 | 0.322542 |
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Target: 5'- aGUCAGGAaaucGGGACGCgaaucgccGGgACGCCGUAu -3' miRNA: 3'- -UAGUCUUc---CCCUGCG--------CCgUGUGGCAU- -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 4109 | 0.69 | 0.284547 |
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Target: 5'- -gCGGuucGGGGACGCGGCG-GCCGa- -3' miRNA: 3'- uaGUCuu-CCCCUGCGCCGUgUGGCau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 19628 | 0.7 | 0.263496 |
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Target: 5'- -cUAGGAGGGG-CGCGGCGC-UCGa- -3' miRNA: 3'- uaGUCUUCCCCuGCGCCGUGuGGCau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 33869 | 0.7 | 0.239924 |
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Target: 5'- ---cGAAGGGGgcgaacgcagccgagGCGCGGCAgGCCGg- -3' miRNA: 3'- uaguCUUCCCC---------------UGCGCCGUgUGGCau -5' |
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| 33176 | 5' | -58.4 | NC_007497.1 | + | 41638 | 1.04 | 0.000707 |
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Target: 5'- cAUCAGAAGGGGACGCGGCACACCGUAc -3' miRNA: 3'- -UAGUCUUCCCCUGCGCCGUGUGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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