Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33177 | 5' | -51.7 | NC_007497.1 | + | 32898 | 0.67 | 0.752356 |
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Target: 5'- gAUCAgcGGCUCacgUUGAGCgCgAUUGGCGUg -3' miRNA: 3'- -UAGU--UCGAG---AACUUGaGgUAGCCGCA- -5' |
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| 33177 | 5' | -51.7 | NC_007497.1 | + | 41204 | 0.67 | 0.741349 |
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Target: 5'- uUCGA--UCUacGACUCCAUCGGCGa -3' miRNA: 3'- uAGUUcgAGAacUUGAGGUAGCCGCa -5' |
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| 33177 | 5' | -51.7 | NC_007497.1 | + | 16339 | 0.68 | 0.716719 |
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Target: 5'- ----cGCUgUUGAACggagcgcgccggCCGUCGGCGUa -3' miRNA: 3'- uaguuCGAgAACUUGa-----------GGUAGCCGCA- -5' |
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| 33177 | 5' | -51.7 | NC_007497.1 | + | 35505 | 0.69 | 0.649984 |
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Target: 5'- gAUCAGGUUCUcgacggUGAGCUugCCGuccUCGGCGa -3' miRNA: 3'- -UAGUUCGAGA------ACUUGA--GGU---AGCCGCa -5' |
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| 33177 | 5' | -51.7 | NC_007497.1 | + | 29681 | 0.69 | 0.649984 |
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Target: 5'- uUCAGGCg---GAcgGCUCgAUCGGCGUc -3' miRNA: 3'- uAGUUCGagaaCU--UGAGgUAGCCGCA- -5' |
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| 33177 | 5' | -51.7 | NC_007497.1 | + | 42189 | 1.05 | 0.00273 |
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Target: 5'- cAUCAAGCUCUUGAACUCCAUCGGCGUc -3' miRNA: 3'- -UAGUUCGAGAACUUGAGGUAGCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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