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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 33180 | 5' | -48.1 | NC_007501.1 | + | 32260 | 0.66 | 0.909315 |
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Target: 5'- cUCCgAGCC-UCAGUGAugauaaaGCUg--UGGCa -3' miRNA: 3'- aAGG-UCGGuAGUCAUU-------UGAagaACCG- -5' |
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| 33180 | 5' | -48.1 | NC_007501.1 | + | 18364 | 0.67 | 0.870118 |
|
Target: 5'- -gCCAG-CAUCAG-GAACgaCUUGGCc -3' miRNA: 3'- aaGGUCgGUAGUCaUUUGaaGAACCG- -5' |
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| 33180 | 5' | -48.1 | NC_007501.1 | + | 10761 | 0.69 | 0.801935 |
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Target: 5'- -gCCAGCUAaUAGUuGGCUagguggUCUUGGCu -3' miRNA: 3'- aaGGUCGGUaGUCAuUUGA------AGAACCG- -5' |
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| 33180 | 5' | -48.1 | NC_007501.1 | + | 24273 | 0.71 | 0.675416 |
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Target: 5'- aUUCCAGaCCAUaagaAGUGGGCUgaUCUUgcGGCg -3' miRNA: 3'- -AAGGUC-GGUAg---UCAUUUGA--AGAA--CCG- -5' |
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| 33180 | 5' | -48.1 | NC_007501.1 | + | 6492 | 0.71 | 0.66331 |
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Target: 5'- -aCCAGCC-UUAGUAAuCUucUCUUGGUc -3' miRNA: 3'- aaGGUCGGuAGUCAUUuGA--AGAACCG- -5' |
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| 33180 | 5' | -48.1 | NC_007501.1 | + | 10784 | 1.12 | 0.001716 |
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Target: 5'- aUUCCAGCCAUCAGUAAACUUCUUGGCg -3' miRNA: 3'- -AAGGUCGGUAGUCAUUUGAAGAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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