miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33182 5' -54.7 NC_007501.1 + 16589 0.67 0.612615
Target:  5'- aCCUuugGGC-CCCaCAUC---ACCGUCUGCg -3'
miRNA:   3'- -GGG---CCGuGGG-GUAGaaaUGGUAGGCG- -5'
33182 5' -54.7 NC_007501.1 + 13374 0.67 0.577843
Target:  5'- aUCCGGCucauCCCCAaaaauuagUUGCUgugguucGUCCGCa -3'
miRNA:   3'- -GGGCCGu---GGGGUaga-----AAUGG-------UAGGCG- -5'
33182 5' -54.7 NC_007501.1 + 35728 0.68 0.523896
Target:  5'- aCCCGcaagucGCACCauuCGUCaugugUGCCGUCUGCu -3'
miRNA:   3'- -GGGC------CGUGGg--GUAGaa---AUGGUAGGCG- -5'
33182 5' -54.7 NC_007501.1 + 17033 1.14 0.000309
Target:  5'- cCCCGGCACCCCAUCUUUACCAUCCGCa -3'
miRNA:   3'- -GGGCCGUGGGGUAGAAAUGGUAGGCG- -5'
33182 5' -54.7 NC_007501.1 + 16820 1.14 0.000309
Target:  5'- cCCCGGCACCCCAUCUUUACCAUCCGCa -3'
miRNA:   3'- -GGGCCGUGGGGUAGAAAUGGUAGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.