miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33184 5' -45.8 NC_007501.1 + 6146 0.76 0.606694
Target:  5'- aCGCGGACACUcacccAGCAGUacacgcUUGUGGGCAc -3'
miRNA:   3'- -GCGCUUGUGGa----UUGUCA------AACACUUGU- -5'
33184 5' -45.8 NC_007501.1 + 17284 0.67 0.976175
Target:  5'- cCGgGAACACCUuGCGGaccUUGUGGc-- -3'
miRNA:   3'- -GCgCUUGUGGAuUGUCa--AACACUugu -5'
33184 5' -45.8 NC_007501.1 + 26321 0.67 0.966103
Target:  5'- aUGUGGACACUUGAUGGacacUUGaUGGACAg -3'
miRNA:   3'- -GCGCUUGUGGAUUGUCa---AAC-ACUUGU- -5'
33184 5' -45.8 NC_007501.1 + 30208 1.11 0.004873
Target:  5'- uCGCGAACACCUAACAGUUUGUGAACAa -3'
miRNA:   3'- -GCGCUUGUGGAUUGUCAAACACUUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.