miRNA: 3'- cGUAGGGGAGCuaCUGCGG--GCUCCUC- -5'
miRNA: 3'- -CGUAGg----GGAGCUACUgCGG--GCUCCuc -5'
miRNA: 3'- -CGUAGGGGAGCUACUGCGGGCUCCUC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33191 | 3' | -57.9 | NC_007518.1 | + | 2079 | 0.68 | 0.047663 |
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Target: 5'- cCAUCUCCUCG--GGCGUCauCGAGGGGa -3' miRNA: 3'- cGUAGGGGAGCuaCUGCGG--GCUCCUC- -5' |
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| 33191 | 3' | -57.9 | NC_007518.1 | + | 2297 | 0.69 | 0.042949 |
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Target: 5'- gGCAUCaaacgCCUCGGUGAgGCCauCGAGGc- -3' miRNA: 3'- -CGUAGg----GGAGCUACUgCGG--GCUCCuc -5' |
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| 33191 | 3' | -57.9 | NC_007518.1 | + | 2045 | 1.11 | 8e-06 |
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Target: 5'- cGCAUCCCCUCGAUGACGCCCGAGGAGa -3' miRNA: 3'- -CGUAGGGGAGCUACUGCGGGCUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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