miRNA: 3'- aUGC-CU---CCaCGaGCGUUUGCGAGAUG- -5'
miRNA: 3'- aUGCCUCCACgAGCGuuUgCGAGAUG- -5'
miRNA: 3'- -AUGCCUC-CACG-AGCGUUugCGagaUG- -5'
miRNA: 3'- aUGCCUCCAC-GAgCGUUugCgaGAUG- -5'
miRNA: 3'- -AUGCCUCCACGAGCGUUUGCGAGAUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 332 | 3' | -53.9 | AC_000010.1 | + | 24423 | 0.66 | 0.602331 |
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Target: 5'- gAUGaGAcucGG-GC-CGCAAAUGCUCUGCa -3' miRNA: 3'- aUGC-CU---CCaCGaGCGUUUGCGAGAUG- -5' |
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| 332 | 3' | -53.9 | AC_000010.1 | + | 16976 | 0.67 | 0.579191 |
|
Target: 5'- aAUGGAGGUGgUUGU--GgGCUUUGCa -3' miRNA: 3'- aUGCCUCCACgAGCGuuUgCGAGAUG- -5' |
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| 332 | 3' | -53.9 | AC_000010.1 | + | 17212 | 0.67 | 0.567689 |
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Target: 5'- gUGCGGAGaGUGUaUCGCGAugGCcgcGCg -3' miRNA: 3'- -AUGCCUC-CACG-AGCGUUugCGagaUG- -5' |
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| 332 | 3' | -53.9 | AC_000010.1 | + | 23518 | 0.69 | 0.436515 |
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Target: 5'- gGCGGAGGUGaCU-GCGAugGg-CUGCg -3' miRNA: 3'- aUGCCUCCAC-GAgCGUUugCgaGAUG- -5' |
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| 332 | 3' | -53.9 | AC_000010.1 | + | 15677 | 1.09 | 0.000656 |
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Target: 5'- gUACGGAGGUGCUCGCAAACGCUCUACa -3' miRNA: 3'- -AUGCCUCCACGAGCGUUUGCGAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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