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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
332 | 5' | -56.2 | AC_000010.1 | + | 15711 | 1.12 | 0.00027 |
Target: 5'- cCUGUGCGAGUGCGCGGACACUUCCGCg -3' miRNA: 3'- -GACACGCUCACGCGCCUGUGAAGGCG- -5' |
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332 | 5' | -56.2 | AC_000010.1 | + | 3277 | 0.69 | 0.384956 |
Target: 5'- --aUGCGAGUGCGgGGGCAaacaUGCc -3' miRNA: 3'- gacACGCUCACGCgCCUGUgaagGCG- -5' |
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332 | 5' | -56.2 | AC_000010.1 | + | 7325 | 0.68 | 0.403537 |
Target: 5'- --uUGCGGGUGaUGCGGaaaggcugggGCACUUuuGCu -3' miRNA: 3'- gacACGCUCAC-GCGCC----------UGUGAAggCG- -5' |
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332 | 5' | -56.2 | AC_000010.1 | + | 18082 | 0.68 | 0.403537 |
Target: 5'- -aGUGUcuccuccagaucGGG-GCGCGGACGCUUgUCGCc -3' miRNA: 3'- gaCACG------------CUCaCGCGCCUGUGAA-GGCG- -5' |
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332 | 5' | -56.2 | AC_000010.1 | + | 13691 | 0.73 | 0.202426 |
Target: 5'- cCUGUGCucGUGCGCGuACGuCUUCCaGCg -3' miRNA: 3'- -GACACGcuCACGCGCcUGU-GAAGG-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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