miRNA: 3'- gGCGGCga--CCG-CGUgAGCGGUGGGUG- -5'
miRNA: 3'- -GGCGgCGACCG-CGUGag-CGgUGG-GUG- -5'
miRNA: 3'- ggCGGCGACcGCGUGaGCGGUgGGUG- -5'
miRNA: 3'- -GGCGGCGACCGCGUGAGCGGUGGGUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33218 | 3' | -63 | NC_007523.1 | + | 992 | 0.67 | 0.029768 |
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Target: 5'- gCGCUGagacGGCaGCGgUCGUUGCCCACa -3' miRNA: 3'- gGCGGCga--CCG-CGUgAGCGGUGGGUG- -5' |
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| 33218 | 3' | -63 | NC_007523.1 | + | 2925 | 0.7 | 0.014121 |
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Target: 5'- cUCGCgGCUGGCgGCACgcaGCgGCCuCGCu -3' miRNA: 3'- -GGCGgCGACCG-CGUGag-CGgUGG-GUG- -5' |
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| 33218 | 3' | -63 | NC_007523.1 | + | 2954 | 0.72 | 0.009709 |
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Target: 5'- -gGCCGCUG-CGUGC-CGCCAgCCGCg -3' miRNA: 3'- ggCGGCGACcGCGUGaGCGGUgGGUG- -5' |
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| 33218 | 3' | -63 | NC_007523.1 | + | 938 | 1.11 | 2e-06 |
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Target: 5'- cCCGCCGCUGGCGCACUCGCCACCCACg -3' miRNA: 3'- -GGCGGCGACCGCGUGAGCGGUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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