Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33264 | 3' | -52.1 | NC_007605.1 | + | 5243 | 0.66 | 0.991884 |
Target: 5'- -aCGCAGgGCGGGgaaACGAUUaaucAGCg -3' miRNA: 3'- gaGCGUCgCGCCUg--UGCUAGaaa-UCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 160501 | 0.66 | 0.991884 |
Target: 5'- --aGCAGCGCGG-CAgaggccucucuCGAguUCUcUAGCa -3' miRNA: 3'- gagCGUCGCGCCuGU-----------GCU--AGAaAUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 58516 | 0.66 | 0.990719 |
Target: 5'- --gGCGGCGgGGACGCccGGUgCUgaUAGCa -3' miRNA: 3'- gagCGUCGCgCCUGUG--CUA-GAa-AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 128149 | 0.66 | 0.990719 |
Target: 5'- -gCGCGGCGgGGGCAUugcccagCUUgGGCa -3' miRNA: 3'- gaGCGUCGCgCCUGUGcua----GAAaUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 109629 | 0.66 | 0.989426 |
Target: 5'- -cUGCGGUGUgaGGACACGGUCa----- -3' miRNA: 3'- gaGCGUCGCG--CCUGUGCUAGaaaucg -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 10186 | 0.66 | 0.989426 |
Target: 5'- -gCGCAG-GCGGAgccgUAgGGUCUUUAGa -3' miRNA: 3'- gaGCGUCgCGCCU----GUgCUAGAAAUCg -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 22148 | 0.66 | 0.987996 |
Target: 5'- -gCGCcaGGCGCgGGGC-CGGUCggcUGGCu -3' miRNA: 3'- gaGCG--UCGCG-CCUGuGCUAGaa-AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 157294 | 0.66 | 0.987845 |
Target: 5'- uCUgGCGGCGCaGGGagucguaggcaaaUugGAUCUggacggUGGCg -3' miRNA: 3'- -GAgCGUCGCG-CCU-------------GugCUAGAa-----AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 58182 | 0.66 | 0.984693 |
Target: 5'- -aCGaCGGCGgGGAguUGGUCUUU-GCa -3' miRNA: 3'- gaGC-GUCGCgCCUguGCUAGAAAuCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 45109 | 0.67 | 0.982802 |
Target: 5'- uCUCGCAGaGUGGGCAgaUGGUCcgugaGGCa -3' miRNA: 3'- -GAGCGUCgCGCCUGU--GCUAGaaa--UCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 112548 | 0.67 | 0.978499 |
Target: 5'- cCUCGCAGCGCGG-C-CG-UCUgaccgAGa -3' miRNA: 3'- -GAGCGUCGCGCCuGuGCuAGAaa---UCg -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 52283 | 0.67 | 0.97607 |
Target: 5'- gCUCG-GGCGCGGGC-CGuaCUUUGGUu -3' miRNA: 3'- -GAGCgUCGCGCCUGuGCuaGAAAUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 74854 | 0.67 | 0.973446 |
Target: 5'- gCUCGcCAGCGgucCGGGCACucaaaGGUCUccGGCu -3' miRNA: 3'- -GAGC-GUCGC---GCCUGUG-----CUAGAaaUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 74077 | 0.68 | 0.97062 |
Target: 5'- -aUGCGGCGCaGACagcccACGAUCUUgggacGCa -3' miRNA: 3'- gaGCGUCGCGcCUG-----UGCUAGAAau---CG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 65307 | 0.68 | 0.957162 |
Target: 5'- uCUgGCGGUGCGGGCACag-Cg--GGCc -3' miRNA: 3'- -GAgCGUCGCGCCUGUGcuaGaaaUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 2860 | 0.69 | 0.953231 |
Target: 5'- -cCGgGGC-CGGugGCGGUCUggUAGUa -3' miRNA: 3'- gaGCgUCGcGCCugUGCUAGAa-AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 156164 | 0.69 | 0.949063 |
Target: 5'- -gUGCGGCgGCGGucuCugGAUCgugaUAGCg -3' miRNA: 3'- gaGCGUCG-CGCCu--GugCUAGaa--AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 77178 | 0.69 | 0.944656 |
Target: 5'- --aGCGGgaGCGGGCACG-UCgggUGGCg -3' miRNA: 3'- gagCGUCg-CGCCUGUGCuAGaa-AUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 61440 | 0.71 | 0.900583 |
Target: 5'- gUCGCGGCGgGGGCgGCGG-Cg--GGCg -3' miRNA: 3'- gAGCGUCGCgCCUG-UGCUaGaaaUCG- -5' |
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33264 | 3' | -52.1 | NC_007605.1 | + | 159034 | 0.71 | 0.893976 |
Target: 5'- -gCGCcGgGCGGACGCGGUC---AGCc -3' miRNA: 3'- gaGCGuCgCGCCUGUGCUAGaaaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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