Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33267 | 5' | -53.6 | NC_007605.1 | + | 105267 | 0.66 | 0.966414 |
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Target: 5'- gCCCGUCgcgugUCUGAGGUGCcauaGCGgUGAa -3' miRNA: 3'- -GGGCAGa----AGACUUCGUGac--CGC-ACUa -5' |
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| 33267 | 5' | -53.6 | NC_007605.1 | + | 151598 | 0.67 | 0.955776 |
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Target: 5'- gCCCGUCUcggcCUacGAGGCcCUGGcCGUGGc -3' miRNA: 3'- -GGGCAGAa---GA--CUUCGuGACC-GCACUa -5' |
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| 33267 | 5' | -53.6 | NC_007605.1 | + | 155649 | 0.67 | 0.951773 |
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Target: 5'- uCCaCGUCcagaccCUGAGGCgcGCUGGCGUaGAa -3' miRNA: 3'- -GG-GCAGaa----GACUUCG--UGACCGCA-CUa -5' |
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| 33267 | 5' | -53.6 | NC_007605.1 | + | 151571 | 0.67 | 0.947533 |
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Target: 5'- aCC--CUUCUGAAGUAuCUGGCGgUGAc -3' miRNA: 3'- gGGcaGAAGACUUCGU-GACCGC-ACUa -5' |
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| 33267 | 5' | -53.6 | NC_007605.1 | + | 73684 | 0.69 | 0.884882 |
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Target: 5'- uCCC-UCgu-UGAAGCACUGuGCGUGGg -3' miRNA: 3'- -GGGcAGaagACUUCGUGAC-CGCACUa -5' |
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| 33267 | 5' | -53.6 | NC_007605.1 | + | 4374 | 1.07 | 0.006858 |
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Target: 5'- gCCCGUCUUCUGAAGCACUGGCGUGAUc -3' miRNA: 3'- -GGGCAGAAGACUUCGUGACCGCACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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