Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33268 | 3' | -55.8 | NC_007605.1 | + | 49015 | 0.66 | 0.892159 |
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Target: 5'- cGGGCGCUCGgug-GCUCaGCcagccucUGGCAGc -3' miRNA: 3'- -UCCGUGAGCaugaCGAG-CG-------ACCGUUu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 149570 | 0.67 | 0.863914 |
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Target: 5'- cAGGCACUCGUcCUG---GCUGGUGAc -3' miRNA: 3'- -UCCGUGAGCAuGACgagCGACCGUUu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 160691 | 0.67 | 0.839938 |
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Target: 5'- gAGGC-CUCugccgcGCUGCUCGCcccUGGUAGAa -3' miRNA: 3'- -UCCGuGAGca----UGACGAGCG---ACCGUUU- -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 55022 | 0.67 | 0.831546 |
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Target: 5'- aAGGC-CUCGaGCUGUuccUCGgUGGCGGg -3' miRNA: 3'- -UCCGuGAGCaUGACG---AGCgACCGUUu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 161034 | 0.69 | 0.768027 |
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Target: 5'- cAGGC-CUCGgccggGCUGCU-GCUGGguGGa -3' miRNA: 3'- -UCCGuGAGCa----UGACGAgCGACCguUU- -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 37261 | 0.69 | 0.728778 |
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Target: 5'- uGGCuguGCUgGUGCUGCUgGUggUGGCAAu -3' miRNA: 3'- uCCG---UGAgCAUGACGAgCG--ACCGUUu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 63543 | 0.7 | 0.667505 |
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Target: 5'- gGGGCugUCGUACaGCagGUUGGCc-- -3' miRNA: 3'- -UCCGugAGCAUGaCGagCGACCGuuu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 61542 | 0.71 | 0.615534 |
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Target: 5'- cGGGCgaagcgGCUCGUGggGCUCGCggugGGCAGc -3' miRNA: 3'- -UCCG------UGAGCAUgaCGAGCGa---CCGUUu -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 51037 | 0.71 | 0.615534 |
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Target: 5'- gGGGCugUUGUGgUGCUgGUUGGgGAAg -3' miRNA: 3'- -UCCGugAGCAUgACGAgCGACCgUUU- -5' |
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| 33268 | 3' | -55.8 | NC_007605.1 | + | 4887 | 1.06 | 0.003947 |
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Target: 5'- cAGGCACUCGUACUGCUCGCUGGCAAAg -3' miRNA: 3'- -UCCGUGAGCAUGACGAGCGACCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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