Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33271 | 5' | -57 | NC_007605.1 | + | 140686 | 0.66 | 0.862687 |
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Target: 5'- cGGCGGGggguggccGGCUGCAGCCGgGucCGgGUUc -3' miRNA: 3'- -CUGCCCa-------CCGAUGUCGGUgU--GUgCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 141705 | 0.66 | 0.891097 |
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Target: 5'- cGGCGGGgggugccGGCUGCAGCCGgGuC-CGg- -3' miRNA: 3'- -CUGCCCa------CCGAUGUCGGUgU-GuGCag -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 143368 | 0.66 | 0.862687 |
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Target: 5'- aGCGGGUGGC-GCGgggucccuGCCugGCACcaggGUCc -3' miRNA: 3'- cUGCCCACCGaUGU--------CGGugUGUG----CAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 150925 | 0.66 | 0.877322 |
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Target: 5'- gGACGuGGUcGGCUGgcaccaGGCCugGcCAUGUCa -3' miRNA: 3'- -CUGC-CCA-CCGAUg-----UCGGugU-GUGCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 151298 | 0.68 | 0.787476 |
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Target: 5'- cGAgGGGcaGGCUGCGGCCGCcCAgGg- -3' miRNA: 3'- -CUgCCCa-CCGAUGUCGGUGuGUgCag -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 161654 | 0.67 | 0.831011 |
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Target: 5'- cGACcacGGUGGuCUgcGCGGCCACAgACuGUCu -3' miRNA: 3'- -CUGc--CCACC-GA--UGUCGGUGUgUG-CAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 163005 | 0.67 | 0.822629 |
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Target: 5'- aGACGGGcagcuacgUGGCggggGCGGCCGC-CA-GUCc -3' miRNA: 3'- -CUGCCC--------ACCGa---UGUCGGUGuGUgCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 163665 | 0.68 | 0.805362 |
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Target: 5'- -cCGGGcUGGC-GCAGgCGCuGCGCGUCc -3' miRNA: 3'- cuGCCC-ACCGaUGUCgGUG-UGUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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