Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33274 | 5' | -49.2 | NC_007605.1 | + | 152966 | 0.67 | 0.994337 |
|
Target: 5'- --aAGGGCCCCCUUccuuuCCCUu-- -3' miRNA: 3'- auaUUCCGGGGGAAcaauuGGGAuuu -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 154152 | 0.69 | 0.978515 |
|
Target: 5'- --cAAGGCCUUCgUGgaGGCCCUGAGc -3' miRNA: 3'- auaUUCCGGGGGaACaaUUGGGAUUU- -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 154823 | 0.66 | 0.997944 |
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Target: 5'- gAUGAGGCCggugaugcggaCCUUggUGUUGGCCCg--- -3' miRNA: 3'- aUAUUCCGG-----------GGGA--ACAAUUGGGauuu -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 161145 | 0.66 | 0.995879 |
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Target: 5'- aUAUAAuGGCCUCCagGccAGCCCUGAu -3' miRNA: 3'- -AUAUU-CCGGGGGaaCaaUUGGGAUUu -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 162129 | 0.73 | 0.886953 |
|
Target: 5'- cGUGAGGaCCCCCUUGUcgaUGGCCa---- -3' miRNA: 3'- aUAUUCC-GGGGGAACA---AUUGGgauuu -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 163923 | 0.66 | 0.997534 |
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Target: 5'- gGUcGGGCCUCCU-GUgcagAGCCUUGAc -3' miRNA: 3'- aUAuUCCGGGGGAaCAa---UUGGGAUUu -5' |
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| 33274 | 5' | -49.2 | NC_007605.1 | + | 168979 | 0.66 | 0.997534 |
|
Target: 5'- ----cGGCCCCCUcGagGACCCc--- -3' miRNA: 3'- auauuCCGGGGGAaCaaUUGGGauuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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