Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33275 | 5' | -49.6 | NC_007605.1 | + | 44934 | 0.66 | 0.997068 |
Target: 5'- -gUGCACAUCUGcAGcCAGGCccuuaGCCu -3' miRNA: 3'- ugACGUGUAGAUaUUuGUCCGa----CGGu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 163650 | 0.66 | 0.996526 |
Target: 5'- cGCUGCGCGUCcgacggGUGGGCAagcuggugGaGCUGCUg -3' miRNA: 3'- -UGACGUGUAGa-----UAUUUGU--------C-CGACGGu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 105893 | 0.66 | 0.995195 |
Target: 5'- cCUGCcCAUCcauUAUGuggGACGGGCgagGCCGg -3' miRNA: 3'- uGACGuGUAG---AUAU---UUGUCCGa--CGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 149738 | 0.67 | 0.994724 |
Target: 5'- -aUGUACcaGguaaacuucccuuucUCUAUGAACAGGCUGCgCGg -3' miRNA: 3'- ugACGUG--U---------------AGAUAUUUGUCCGACG-GU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 48789 | 0.67 | 0.990012 |
Target: 5'- aACUGCGgcUCUGc-GGCgguGGGCUGCCAg -3' miRNA: 3'- -UGACGUguAGAUauUUG---UCCGACGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 121126 | 0.67 | 0.990012 |
Target: 5'- aACUGCACcUCUGUGuugcugguGGCuGGgUGCCc -3' miRNA: 3'- -UGACGUGuAGAUAU--------UUGuCCgACGGu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 63845 | 0.68 | 0.988582 |
Target: 5'- --cGCGCAUCggcgGGGCAcGGCUGCa- -3' miRNA: 3'- ugaCGUGUAGaua-UUUGU-CCGACGgu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 153815 | 0.68 | 0.988582 |
Target: 5'- cCUGCAaGUUUGUGcaGACAcGCUGCCGg -3' miRNA: 3'- uGACGUgUAGAUAU--UUGUcCGACGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 161658 | 0.68 | 0.986997 |
Target: 5'- uCUGCuugGCGUaagu-GACAGGUUGCCAg -3' miRNA: 3'- uGACG---UGUAgauauUUGUCCGACGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 86205 | 0.7 | 0.965263 |
Target: 5'- uGCaGCGCAUCaggcgaaggcggagaAGACGGGCUGCCu -3' miRNA: 3'- -UGaCGUGUAGaua------------UUUGUCCGACGGu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 129008 | 0.7 | 0.96422 |
Target: 5'- cCUGUccacGCAUUUGUAGGCGGGgagaUGCCAc -3' miRNA: 3'- uGACG----UGUAGAUAUUUGUCCg---ACGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 158597 | 0.7 | 0.960584 |
Target: 5'- gGCUGCACcgUgGUGGcgggggguGCGGGCUGCUc -3' miRNA: 3'- -UGACGUGuaGaUAUU--------UGUCCGACGGu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 123887 | 0.71 | 0.932878 |
Target: 5'- cAUUGUACAUCUuuu-GCAGGCUuuccacgGCCAc -3' miRNA: 3'- -UGACGUGUAGAuauuUGUCCGA-------CGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 90129 | 0.73 | 0.874785 |
Target: 5'- uGCUGCcaaauCAUCUGaaaauGACAGGCUGCg- -3' miRNA: 3'- -UGACGu----GUAGAUau---UUGUCCGACGgu -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 3320 | 0.75 | 0.806235 |
Target: 5'- --cGCGCAUgCgauauGGCAGGCUGCCAg -3' miRNA: 3'- ugaCGUGUA-Gauau-UUGUCCGACGGU- -5' |
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33275 | 5' | -49.6 | NC_007605.1 | + | 36147 | 1.09 | 0.011179 |
Target: 5'- uACUGCACAUCUAUAAACAGGCUGCCAc -3' miRNA: 3'- -UGACGUGUAGAUAUUUGUCCGACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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