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miRNA: 3'- uGUAuCGAGUCUAGguCCGUCcGGUc -5'
miRNA: 3'- uguaUCGaGUCUaGGUCCG--------UCCGGUC- -5'
miRNA: 3'- -UGUAUCGAGuc--UAGGuCCGU-CCGGUC- -5'
miRNA: 3'- uguaUCGAGUCuaGGUcCGUCCGGUC- -5'
miRNA: 3'- -UGUA---UCGAGUCUaGGuCCGU---CCGGuc -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 33288 | 5' | -55.7 | NC_007605.1 | + | 46509 | 0.66 | 0.887245 |
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Target: 5'- uCAUuGCUCGGAUUguGGCAG-CCGa -3' miRNA: 3'- uGUAuCGAGUCUAGguCCGUCcGGUc -5' |
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| 33288 | 5' | -55.7 | NC_007605.1 | + | 61936 | 0.66 | 0.894055 |
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Target: 5'- ---aAGC-CGGAgCCGGGCggcgucaaAGGCCAGg -3' miRNA: 3'- uguaUCGaGUCUaGGUCCG--------UCCGGUC- -5' |
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| 33288 | 5' | -55.7 | NC_007605.1 | + | 92066 | 0.66 | 0.900631 |
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Target: 5'- cACGUAGCUCcucuGUCC-GGCGuGGCUGGa -3' miRNA: 3'- -UGUAUCGAGuc--UAGGuCCGU-CCGGUC- -5' |
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| 33288 | 5' | -55.7 | NC_007605.1 | + | 160614 | 0.66 | 0.913066 |
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Target: 5'- ---cAGCUCGG--CCA-GCAGGCCGGc -3' miRNA: 3'- uguaUCGAGUCuaGGUcCGUCCGGUC- -5' |
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| 33288 | 5' | -55.7 | NC_007605.1 | + | 50446 | 0.66 | 0.918921 |
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Target: 5'- uGCAUuccGGCUCGGAgCCuGGCGcgcGGCCc- -3' miRNA: 3'- -UGUA---UCGAGUCUaGGuCCGU---CCGGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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