Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33288 | 5' | -55.7 | NC_007605.1 | + | 61936 | 0.66 | 0.894055 |
Target: 5'- ---aAGC-CGGAgCCGGGCggcgucaaAGGCCAGg -3' miRNA: 3'- uguaUCGaGUCUaGGUCCG--------UCCGGUC- -5' |
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33288 | 5' | -55.7 | NC_007605.1 | + | 46509 | 0.66 | 0.887245 |
Target: 5'- uCAUuGCUCGGAUUguGGCAG-CCGa -3' miRNA: 3'- uGUAuCGAGUCUAGguCCGUCcGGUc -5' |
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33288 | 5' | -55.7 | NC_007605.1 | + | 135067 | 0.67 | 0.872938 |
Target: 5'- gACcUGGCUCAGGUCCGGcgcccugcGCAGuCCAa -3' miRNA: 3'- -UGuAUCGAGUCUAGGUC--------CGUCcGGUc -5' |
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33288 | 5' | -55.7 | NC_007605.1 | + | 6199 | 0.68 | 0.841724 |
Target: 5'- ---gGGCUCGGA---AGGCAGGCgGGg -3' miRNA: 3'- uguaUCGAGUCUaggUCCGUCCGgUC- -5' |
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33288 | 5' | -55.7 | NC_007605.1 | + | 110310 | 0.77 | 0.347279 |
Target: 5'- aGCcgAGCgacugCAGAUCCAGGCgaaGGGCCGu -3' miRNA: 3'- -UGuaUCGa----GUCUAGGUCCG---UCCGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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