Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33289 | 3' | -61.4 | NC_007605.1 | + | 85469 | 0.66 | 0.668341 |
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Target: 5'- aGGa--GgUGGCGUGGGAgUGGGCgCCc -3' miRNA: 3'- -UCgagCgACCGCACUCUgACCCG-GGu -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 95094 | 0.68 | 0.530984 |
|
Target: 5'- gGGCUCGCgggauggGGcCG-GAGGCaGGGCCgAa -3' miRNA: 3'- -UCGAGCGa------CC-GCaCUCUGaCCCGGgU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 131503 | 0.66 | 0.658475 |
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Target: 5'- gAGCUUGCUGGUuaccGAGuCUGGuGCCa- -3' miRNA: 3'- -UCGAGCGACCGca--CUCuGACC-CGGgu -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 140631 | 0.69 | 0.52526 |
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Target: 5'- gAGCUCGggGGCGgccGGGuggcccaccggguccGCUGGGUCCGc -3' miRNA: 3'- -UCGAGCgaCCGCa--CUC---------------UGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 144087 | 0.7 | 0.465857 |
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Target: 5'- cGGggUGCUGGgGUGGGGgaUGGGCUCAg -3' miRNA: 3'- -UCgaGCGACCgCACUCUg-ACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 144616 | 0.69 | 0.521458 |
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Target: 5'- aGGCUCccaaGGCG-GGGGUUGGGCCCAg -3' miRNA: 3'- -UCGAGcga-CCGCaCUCUGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 155666 | 0.7 | 0.465857 |
|
Target: 5'- aGGCgCGCUGGCGUaGAaguaGGCUGcuGCCCAa -3' miRNA: 3'- -UCGaGCGACCGCA-CU----CUGACc-CGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 161125 | 0.66 | 0.696767 |
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Target: 5'- cGCUCGggacuuuCUGGagggaGUGuGGGgUGGGCCCGg -3' miRNA: 3'- uCGAGC-------GACCg----CAC-UCUgACCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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