Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33289 | 3' | -61.4 | NC_007605.1 | + | 16838 | 0.68 | 0.559919 |
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Target: 5'- cGUggGgaGGgGUggacGAGGCUGGGCCCGg -3' miRNA: 3'- uCGagCgaCCgCA----CUCUGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 19060 | 0.68 | 0.559919 |
|
Target: 5'- cGGCUCgGCUGGCc-GAGACccGGGUCUg -3' miRNA: 3'- -UCGAG-CGACCGcaCUCUGa-CCCGGGu -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 19907 | 0.68 | 0.559919 |
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Target: 5'- cGUggGgaGGgGUggacGAGGCUGGGCCCGg -3' miRNA: 3'- uCGagCgaCCgCA----CUCUGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 22128 | 0.68 | 0.559919 |
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Target: 5'- cGGCUgGCUGGCc-GAGACccGGGUCUg -3' miRNA: 3'- -UCGAgCGACCGcaCUCUGa-CCCGGGu -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 22976 | 0.68 | 0.559919 |
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Target: 5'- cGUggGgaGGgGUggacGAGGCUGGGCCCGg -3' miRNA: 3'- uCGagCgaCCgCA----CUCUGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 25197 | 0.68 | 0.559919 |
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Target: 5'- cGGCUCgGCUGGCc-GAGACccGGGUCUg -3' miRNA: 3'- -UCGAG-CGACCGcaCUCUGa-CCCGGGu -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 26045 | 0.68 | 0.559919 |
|
Target: 5'- cGUggGgaGGgGUggacGAGGCUGGGCCCGg -3' miRNA: 3'- uCGagCgaCCgCA----CUCUGACCCGGGU- -5' |
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| 33289 | 3' | -61.4 | NC_007605.1 | + | 48386 | 1.08 | 0.001092 |
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Target: 5'- gAGCUCGCUGGCGUGAGACUGGGCCCAg -3' miRNA: 3'- -UCGAGCGACCGCACUCUGACCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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