Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 33306 | 5' | -62.2 | NC_007605.1 | + | 58324 | 0.66 | 0.649969 |
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Target: 5'- aGCACCGgGcGUCCcCGCCGccUCCCCa- -3' miRNA: 3'- cUGUGGCgC-CGGGaGUGGU--AGGGGca -5' |
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| 33306 | 5' | -62.2 | NC_007605.1 | + | 81485 | 0.66 | 0.659704 |
|
Target: 5'- uAUugUcCGGCCCUgggaGCCAUCCCUGa -3' miRNA: 3'- cUGugGcGCCGGGAg---UGGUAGGGGCa -5' |
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| 33306 | 5' | -62.2 | NC_007605.1 | + | 149466 | 0.66 | 0.669419 |
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Target: 5'- -uUACCGCGGCguauggcguuuaCCggacaCACCuUCCCCGg -3' miRNA: 3'- cuGUGGCGCCG------------GGa----GUGGuAGGGGCa -5' |
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| 33306 | 5' | -62.2 | NC_007605.1 | + | 53984 | 0.66 | 0.678139 |
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Target: 5'- --uGCCGCcaucucgugGGCCUUCGCCAUCagggucuggcucuCCCGg -3' miRNA: 3'- cugUGGCG---------CCGGGAGUGGUAG-------------GGGCa -5' |
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| 33306 | 5' | -62.2 | NC_007605.1 | + | 87446 | 0.66 | 0.688758 |
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Target: 5'- aGugACCGUGgaGCCCgccCACCcgaCCCCGg -3' miRNA: 3'- -CugUGGCGC--CGGGa--GUGGua-GGGGCa -5' |
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| 33306 | 5' | -62.2 | NC_007605.1 | + | 60405 | 0.66 | 0.692607 |
|
Target: 5'- gGGCAUCGCggaucacccccucugGGCCCaCACCAgcgagCCCCu- -3' miRNA: 3'- -CUGUGGCG---------------CCGGGaGUGGUa----GGGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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