Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33306 | 5' | -62.2 | NC_007605.1 | + | 157636 | 0.66 | 0.679106 |
Target: 5'- -cCAuCCGCGGCCCgcgggagCACCcccgCCgCCGUu -3' miRNA: 3'- cuGU-GGCGCCGGGa------GUGGua--GG-GGCA- -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 50889 | 0.66 | 0.688758 |
Target: 5'- --aGCCGCggagauucGGCCCUcCGCCAcccgccUCCCCa- -3' miRNA: 3'- cugUGGCG--------CCGGGA-GUGGU------AGGGGca -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 60492 | 0.66 | 0.688758 |
Target: 5'- gGACGCCGCcgccaggugGGCCCUgGCCAcggCCGa -3' miRNA: 3'- -CUGUGGCG---------CCGGGAgUGGUaggGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 152915 | 0.66 | 0.688758 |
Target: 5'- aGACAgCCGCGGUUgUCAUCAUCaUCGg -3' miRNA: 3'- -CUGU-GGCGCCGGgAGUGGUAGgGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 87446 | 0.66 | 0.688758 |
Target: 5'- aGugACCGUGgaGCCCgccCACCcgaCCCCGg -3' miRNA: 3'- -CugUGGCGC--CGGGa--GUGGua-GGGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 60405 | 0.66 | 0.692607 |
Target: 5'- gGGCAUCGCggaucacccccucugGGCCCaCACCAgcgagCCCCu- -3' miRNA: 3'- -CUGUGGCG---------------CCGGGaGUGGUa----GGGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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