Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33306 | 5' | -62.2 | NC_007605.1 | + | 58304 | 0.69 | 0.487695 |
Target: 5'- cGGCcuCCGCGGCCCcgGCCuccgcggCCCCGg -3' miRNA: 3'- -CUGu-GGCGCCGGGagUGGua-----GGGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 58259 | 0.69 | 0.487695 |
Target: 5'- cGGCcuCCGCGGCCCcgGCCuccgcggCCCCGg -3' miRNA: 3'- -CUGu-GGCGCCGGGagUGGua-----GGGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 50578 | 0.69 | 0.49682 |
Target: 5'- gGGCGCUgGCGGCCggCGcCCGUCCUCGg -3' miRNA: 3'- -CUGUGG-CGCCGGgaGU-GGUAGGGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 154121 | 0.68 | 0.534039 |
Target: 5'- aAC-CUGCaGGCCCUgGCCcccUCCCCGg -3' miRNA: 3'- cUGuGGCG-CCGGGAgUGGu--AGGGGCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 104928 | 0.68 | 0.543502 |
Target: 5'- aGAC-CCGaggcagacaaGGCCC-CGCCAUCCCaCGa -3' miRNA: 3'- -CUGuGGCg---------CCGGGaGUGGUAGGG-GCa -5' |
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33306 | 5' | -62.2 | NC_007605.1 | + | 55855 | 1.06 | 0.001462 |
Target: 5'- cGACACCGCGGCCCUCACCAUCCCCGUc -3' miRNA: 3'- -CUGUGGCGCCGGGAGUGGUAGGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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