Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
33309 | 3' | -48.1 | NC_007605.1 | + | 58173 | 0.76 | 0.865198 |
Target: 5'- aGGCCG-GAGaCGACGgcGGGGAGUUg- -3' miRNA: 3'- -CCGGCuCUC-GCUGCuaCCUUUUAAac -5' |
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33309 | 3' | -48.1 | NC_007605.1 | + | 157465 | 0.77 | 0.832108 |
Target: 5'- gGGCUGGGGGCGAUGGacugcUGGggGGUg-- -3' miRNA: 3'- -CCGGCUCUCGCUGCU-----ACCuuUUAaac -5' |
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33309 | 3' | -48.1 | NC_007605.1 | + | 63606 | 0.82 | 0.57859 |
Target: 5'- cGCCGGGGGUGGCGGUGGAGu----- -3' miRNA: 3'- cCGGCUCUCGCUGCUACCUUuuaaac -5' |
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33309 | 3' | -48.1 | NC_007605.1 | + | 56713 | 1.12 | 0.012803 |
Target: 5'- uGGCCGAGAGCGACGAUGGAAAAUUUGa -3' miRNA: 3'- -CCGGCUCUCGCUGCUACCUUUUAAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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