Results 81 - 86 of 86 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33313 | 5' | -60.8 | NC_007605.1 | + | 87874 | 0.74 | 0.318881 |
Target: 5'- uUCCgcugggCCUCCUGCCGCgggGCCUCACa- -3' miRNA: 3'- uAGGa-----GGGGGGCGGUGa--UGGAGUGcc -5' |
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33313 | 5' | -60.8 | NC_007605.1 | + | 102525 | 0.74 | 0.325917 |
Target: 5'- cUCUUCCCCCCgGCCAUcuuUGCCggaACGGu -3' miRNA: 3'- uAGGAGGGGGG-CGGUG---AUGGag-UGCC- -5' |
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33313 | 5' | -60.8 | NC_007605.1 | + | 13114 | 0.74 | 0.298463 |
Target: 5'- cUCCUCCCCUCcaggcccgaGCCuCUcCCUCGCGGa -3' miRNA: 3'- uAGGAGGGGGG---------CGGuGAuGGAGUGCC- -5' |
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33313 | 5' | -60.8 | NC_007605.1 | + | 3460 | 0.74 | 0.298463 |
Target: 5'- gAUCUUCUCCCC-UCGCUGCCUCACu- -3' miRNA: 3'- -UAGGAGGGGGGcGGUGAUGGAGUGcc -5' |
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33313 | 5' | -60.8 | NC_007605.1 | + | 31528 | 0.74 | 0.298463 |
Target: 5'- cUCCUCCCCUCcaggcccgaGCCuCUcCCUCGCGGa -3' miRNA: 3'- uAGGAGGGGGG---------CGGuGAuGGAGUGCC- -5' |
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33313 | 5' | -60.8 | NC_007605.1 | + | 60568 | 0.97 | 0.008887 |
Target: 5'- cAUCCUCCCCCCGCCACUACUcCACGGa -3' miRNA: 3'- -UAGGAGGGGGGCGGUGAUGGaGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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