Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33313 | 5' | -60.8 | NC_007605.1 | + | 134575 | 0.66 | 0.73961 |
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Target: 5'- -cCCUggggcgCCCCUCGCCcauGCUugCggCGCGGg -3' miRNA: 3'- uaGGA------GGGGGGCGG---UGAugGa-GUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 61411 | 0.66 | 0.748913 |
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Target: 5'- gGUCCgccgccgCCCCCacacCCACaGCCgUCGCGGc -3' miRNA: 3'- -UAGGa------GGGGGgc--GGUGaUGG-AGUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 143384 | 0.66 | 0.748913 |
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Target: 5'- -gCCUCCCCCC-CCAUgaaGCggguggCGCGGg -3' miRNA: 3'- uaGGAGGGGGGcGGUGa--UGga----GUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 40569 | 0.66 | 0.748913 |
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Target: 5'- -gCCUCCCCCC-CCAUgaaGCggguggCGCGGg -3' miRNA: 3'- uaGGAGGGGGGcGGUGa--UGga----GUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 39845 | 0.66 | 0.752608 |
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Target: 5'- -cCCUCCCCCagggcguacccggcuUGCCugguucugggGCU-CCUCugGGg -3' miRNA: 3'- uaGGAGGGGG---------------GCGG----------UGAuGGAGugCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 153438 | 0.66 | 0.758122 |
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Target: 5'- --gCUCCCCCaugggGaCC-CUGCCuUCACGGa -3' miRNA: 3'- uagGAGGGGGg----C-GGuGAUGG-AGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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