Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33313 | 5' | -60.8 | NC_007605.1 | + | 96757 | 0.68 | 0.594164 |
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Target: 5'- cUCCUCgaCCCCGgccuCCACUGCCUCcuCGa -3' miRNA: 3'- uAGGAGg-GGGGC----GGUGAUGGAGu-GCc -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 101000 | 0.68 | 0.643169 |
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Target: 5'- uUCCUCUCCCaCGCCGaggucaagacCUuCCUCACa- -3' miRNA: 3'- uAGGAGGGGG-GCGGU----------GAuGGAGUGcc -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 37391 | 0.68 | 0.647092 |
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Target: 5'- -cCCUCCCCCUGCUcuguccccgGCUcugGCCUUgagucuuagaggguuGCGGg -3' miRNA: 3'- uaGGAGGGGGGCGG---------UGA---UGGAG---------------UGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 151339 | 0.67 | 0.652974 |
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Target: 5'- cUCCUaugcgCCCCCCGUgACggaGCUggggCACGGg -3' miRNA: 3'- uAGGA-----GGGGGGCGgUGa--UGGa---GUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 47646 | 0.67 | 0.662763 |
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Target: 5'- cAUgCUCCCCCCaCCACcgUCUCcCGGg -3' miRNA: 3'- -UAgGAGGGGGGcGGUGauGGAGuGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 60568 | 0.97 | 0.008887 |
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Target: 5'- cAUCCUCCCCCCGCCACUACUcCACGGa -3' miRNA: 3'- -UAGGAGGGGGGCGGUGAUGGaGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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