Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33313 | 5' | -60.8 | NC_007605.1 | + | 132279 | 0.68 | 0.613735 |
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Target: 5'- -aCCUgCCCCUgaGUCACUACCggUugGGg -3' miRNA: 3'- uaGGAgGGGGG--CGGUGAUGGa-GugCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 159767 | 0.68 | 0.613735 |
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Target: 5'- -gCCUUgCCCCCGCCAgCgccuCCUCGCaGGc -3' miRNA: 3'- uaGGAG-GGGGGCGGU-Gau--GGAGUG-CC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 105330 | 0.67 | 0.662763 |
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Target: 5'- -gCCUgCCCUCCGCCAUcaggcguuCC-CACGGg -3' miRNA: 3'- uaGGA-GGGGGGCGGUGau------GGaGUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 131650 | 0.67 | 0.67253 |
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Target: 5'- cUCCUUCCUCUgaGCCuugGCCUgGCGGg -3' miRNA: 3'- uAGGAGGGGGG--CGGugaUGGAgUGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 13834 | 0.67 | 0.682266 |
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Target: 5'- -cCCUCCCgCCCcuguCCACUGCC-C-CGGu -3' miRNA: 3'- uaGGAGGG-GGGc---GGUGAUGGaGuGCC- -5' |
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| 33313 | 5' | -60.8 | NC_007605.1 | + | 87794 | 0.66 | 0.720754 |
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Target: 5'- ----gCCUCCUGCCGCgggGCCUCcugccGCGGg -3' miRNA: 3'- uaggaGGGGGGCGGUGa--UGGAG-----UGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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