miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33318 3' -47.3 NC_007605.1 + 63906 0.66 0.99989
Target:  5'- cGUGGcucuaGCCuccCCGUGGUGGagauugacagcUUUUAUUCc -3'
miRNA:   3'- -CACC-----UGGu--GGCACCACU-----------AAAAUAAGu -5'
33318 3' -47.3 NC_007605.1 + 101463 0.66 0.99989
Target:  5'- aUGGACCACCaaugugaucGUGGUGGa-------- -3'
miRNA:   3'- cACCUGGUGG---------CACCACUaaaauaagu -5'
33318 3' -47.3 NC_007605.1 + 106146 0.66 0.99989
Target:  5'- -gGGGCCaccgGCCGUGGUGGg-------- -3'
miRNA:   3'- caCCUGG----UGGCACCACUaaaauaagu -5'
33318 3' -47.3 NC_007605.1 + 168094 0.69 0.997139
Target:  5'- -gGGAgUCAUCGUGGUGGU---GUUCAu -3'
miRNA:   3'- caCCU-GGUGGCACCACUAaaaUAAGU- -5'
33318 3' -47.3 NC_007605.1 + 84590 0.72 0.983632
Target:  5'- -aGGACCACUGUGG-GGgccugggAUUCAg -3'
miRNA:   3'- caCCUGGUGGCACCaCUaaaa---UAAGU- -5'
33318 3' -47.3 NC_007605.1 + 97005 0.72 0.981556
Target:  5'- cUGGGCCACCuucuUGGUGGU---AUUCAa -3'
miRNA:   3'- cACCUGGUGGc---ACCACUAaaaUAAGU- -5'
33318 3' -47.3 NC_007605.1 + 63682 1.1 0.018012
Target:  5'- cGUGGACCACCGUGGUGAUUUUAUUCAg -3'
miRNA:   3'- -CACCUGGUGGCACCACUAAAAUAAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.