Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33321 | 3' | -47.6 | NC_007605.1 | + | 109841 | 0.66 | 0.999761 |
Target: 5'- gGUUaCGGacaAAGGCC-UCAGUCUCCGGa -3' miRNA: 3'- gCAA-GUCa--UUUUGGcAGUCAGAGGUU- -5' |
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33321 | 3' | -47.6 | NC_007605.1 | + | 3135 | 0.68 | 0.998625 |
Target: 5'- ---aCGGaGGGGCCGUCAGUCcgcUCCAu -3' miRNA: 3'- gcaaGUCaUUUUGGCAGUCAG---AGGUu -5' |
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33321 | 3' | -47.6 | NC_007605.1 | + | 107603 | 0.71 | 0.983632 |
Target: 5'- --gUCAGguacGAGGCCGUCuuuGUCUCCGc -3' miRNA: 3'- gcaAGUCa---UUUUGGCAGu--CAGAGGUu -5' |
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33321 | 3' | -47.6 | NC_007605.1 | + | 66683 | 1.07 | 0.027882 |
Target: 5'- uCGUUCAGUAAAACCGUCAGUCUCCAAc -3' miRNA: 3'- -GCAAGUCAUUUUGGCAGUCAGAGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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