Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33322 | 3' | -51.1 | NC_007605.1 | + | 159057 | 0.66 | 0.990603 |
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Target: 5'- gGUGGGGCAGCgua--CCUGCg---- -3' miRNA: 3'- aCACCUCGUCGaguagGGAUGaauac -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 162359 | 0.66 | 0.987778 |
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Target: 5'- cGUGGuAGCAGCUCuugaccagGUCCUUGuagGUGa -3' miRNA: 3'- aCACC-UCGUCGAG--------UAGGGAUgaaUAC- -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 67857 | 0.67 | 0.982362 |
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Target: 5'- cUGUGGAGaGGCU--UCCCUGCa---- -3' miRNA: 3'- -ACACCUCgUCGAguAGGGAUGaauac -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 110763 | 0.68 | 0.975047 |
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Target: 5'- aUGUGGGGCauggguaGGCUgGUCCUgACUgauUAUGg -3' miRNA: 3'- -ACACCUCG-------UCGAgUAGGGaUGA---AUAC- -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 145877 | 0.68 | 0.971692 |
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Target: 5'- aGUGGGGCuguugAGCUCAacagcccuaagcgcUCCCUACc---- -3' miRNA: 3'- aCACCUCG-----UCGAGU--------------AGGGAUGaauac -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 85093 | 0.7 | 0.937348 |
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Target: 5'- cGUGGGGCAGCUguUUCCg----GUGg -3' miRNA: 3'- aCACCUCGUCGAguAGGGaugaaUAC- -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 88586 | 0.7 | 0.92677 |
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Target: 5'- cGUGGgccGGCGGCUCcugguAUCCCUGg-UAUGg -3' miRNA: 3'- aCACC---UCGUCGAG-----UAGGGAUgaAUAC- -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 43690 | 0.71 | 0.908959 |
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Target: 5'- cUGUaGGcGCAGCUCAUCCCcACa---- -3' miRNA: 3'- -ACA-CCuCGUCGAGUAGGGaUGaauac -5' |
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| 33322 | 3' | -51.1 | NC_007605.1 | + | 67290 | 1.09 | 0.00838 |
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Target: 5'- gUGUGGAGCAGCUCAUCCCUACUUAUGu -3' miRNA: 3'- -ACACCUCGUCGAGUAGGGAUGAAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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