Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33323 | 5' | -48.3 | NC_007605.1 | + | 107025 | 0.66 | 0.99973 |
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Target: 5'- aCGCCUGc-GGCGAGGUggcgCUAAu--- -3' miRNA: 3'- -GCGGGCauCCGUUCCAaa--GAUUuacu -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 150181 | 0.66 | 0.99973 |
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Target: 5'- aGCUCGUcgacGGGCAAGGUgagagUCggggGGcgGGc -3' miRNA: 3'- gCGGGCA----UCCGUUCCAa----AGa---UUuaCU- -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 92612 | 0.66 | 0.999569 |
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Target: 5'- uCGCCCGUGGGCcuuGGccUCauuuaUAAAUGc -3' miRNA: 3'- -GCGGGCAUCCGuu-CCaaAG-----AUUUACu -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 136816 | 0.66 | 0.999461 |
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Target: 5'- aGCCaaaGUAGGCcggaGGGGggUgUGGGUGGg -3' miRNA: 3'- gCGGg--CAUCCG----UUCCaaAgAUUUACU- -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 107358 | 0.67 | 0.998987 |
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Target: 5'- uGCuuGUAGGaAAGGac-CUGGAUGAa -3' miRNA: 3'- gCGggCAUCCgUUCCaaaGAUUUACU- -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 163698 | 0.68 | 0.997414 |
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Target: 5'- cCGCCCccagGUAGGCAgAGGgaUCUAGc--- -3' miRNA: 3'- -GCGGG----CAUCCGU-UCCaaAGAUUuacu -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 88288 | 0.68 | 0.995736 |
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Target: 5'- gGCCCaugaUGGGCGAGGcguuguaggCUGAGUGGc -3' miRNA: 3'- gCGGGc---AUCCGUUCCaaa------GAUUUACU- -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 126273 | 0.69 | 0.991046 |
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Target: 5'- uGCCCGUGGGgccuGGGUUUCg------ -3' miRNA: 3'- gCGGGCAUCCgu--UCCAAAGauuuacu -5' |
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| 33323 | 5' | -48.3 | NC_007605.1 | + | 67993 | 1.1 | 0.015232 |
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Target: 5'- gCGCCCGUAGGCAAGGUUUCUAAAUGAc -3' miRNA: 3'- -GCGGGCAUCCGUUCCAAAGAUUUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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