miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3335 3' -42.8 NC_001547.1 + 11481 1.07 0.003586
Target:  5'- aUAGCAACACUAAAAACUCGAUGUACUu -3'
miRNA:   3'- -AUCGUUGUGAUUUUUGAGCUACAUGA- -5'
3335 3' -42.8 NC_001547.1 + 11171 0.69 0.797583
Target:  5'- cAGCGAUACgaUAAAguucgccugugGACUCGcgGUGCUa -3'
miRNA:   3'- aUCGUUGUG--AUUU-----------UUGAGCuaCAUGA- -5'
3335 3' -42.8 NC_001547.1 + 8160 0.67 0.884061
Target:  5'- cUGGCAACugUGcGAACUCcAUGUcguauGCUg -3'
miRNA:   3'- -AUCGUUGugAUuUUUGAGcUACA-----UGA- -5'
3335 3' -42.8 NC_001547.1 + 2938 0.67 0.900218
Target:  5'- gAGCAACACguucacauGCUCuGAUGUGa- -3'
miRNA:   3'- aUCGUUGUGauuuu---UGAG-CUACAUga -5'
3335 3' -42.8 NC_001547.1 + 11396 0.67 0.910237
Target:  5'- --cCAGCA----AAACUCGAUGUACUu -3'
miRNA:   3'- aucGUUGUgauuUUUGAGCUACAUGA- -5'
3335 3' -42.8 NC_001547.1 + 11573 0.67 0.910237
Target:  5'- cUAGCgGACGCcAAAAACUCaAUGUAUUu -3'
miRNA:   3'- -AUCG-UUGUGaUUUUUGAGcUACAUGA- -5'
3335 3' -42.8 NC_001547.1 + 10408 0.66 0.945325
Target:  5'- -cGCAAUcuGCUGAcAAUUCGAcGUACg -3'
miRNA:   3'- auCGUUG--UGAUUuUUGAGCUaCAUGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.