Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33354 | 5' | -58.6 | NC_007605.1 | + | 140601 | 0.67 | 0.782909 |
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Target: 5'- uGUCCcuuuGGguCUcaggACCCAGCCCUgGAGc -3' miRNA: 3'- -CAGGu---UCguGG----UGGGUCGGGAgCUCu -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 61121 | 0.67 | 0.773782 |
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Target: 5'- aUCCGcgcGCGCCGuuuggCCGGCCCUCaGGGAc -3' miRNA: 3'- cAGGUu--CGUGGUg----GGUCGGGAG-CUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 115240 | 0.67 | 0.772862 |
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Target: 5'- -cCCAccGGCGCUGCCCcggauguugcuggGGUUCUCGAGGg -3' miRNA: 3'- caGGU--UCGUGGUGGG-------------UCGGGAGCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 60508 | 0.67 | 0.7636 |
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Target: 5'- cUCCAAGCugCcCCCGucaagcgcgagauGUCCUcCGAGAg -3' miRNA: 3'- cAGGUUCGugGuGGGU-------------CGGGA-GCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 161276 | 0.68 | 0.697093 |
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Target: 5'- -gCCugcuuGCGCaGCCCAGCCC-CGGGGg -3' miRNA: 3'- caGGuu---CGUGgUGGGUCGGGaGCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 71331 | 0.68 | 0.697093 |
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Target: 5'- cUCUAGGCACC-CUgggGGCCCUUGGGGc -3' miRNA: 3'- cAGGUUCGUGGuGGg--UCGGGAGCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 85237 | 0.69 | 0.667229 |
|
Target: 5'- -cUCAGGUGCCGCCCguuccccggcagAGgCCUCGGGGg -3' miRNA: 3'- caGGUUCGUGGUGGG------------UCgGGAGCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 60141 | 0.69 | 0.647168 |
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Target: 5'- cUCCAgGGCGugcuCCACCCgcucguugAGCuCCUCGAGAu -3' miRNA: 3'- cAGGU-UCGU----GGUGGG--------UCG-GGAGCUCU- -5' |
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| 33354 | 5' | -58.6 | NC_007605.1 | + | 162751 | 0.79 | 0.196951 |
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Target: 5'- uGUUCAGGCACacggccgggGCCCGGCCCUCGcAGAg -3' miRNA: 3'- -CAGGUUCGUGg--------UGGGUCGGGAGC-UCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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