Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33361 | 5' | -55.6 | NC_007605.1 | + | 121260 | 0.71 | 0.684038 |
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Target: 5'- gCCCUGCAGCuuucuGCAGGAGGCauuCCccgCAc -3' miRNA: 3'- aGGGACGUUG-----UGUCCUCCG---GGuuaGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 72973 | 0.71 | 0.663576 |
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Target: 5'- cUCCaGCGAC-CAGGGGGCCCccuuGAUCu -3' miRNA: 3'- aGGGaCGUUGuGUCCUCCGGG----UUAGu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 30121 | 0.71 | 0.653301 |
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Target: 5'- cCUCUGgccCGAUACGGGGGGCCCGGg-- -3' miRNA: 3'- aGGGAC---GUUGUGUCCUCCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 164115 | 0.72 | 0.62241 |
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Target: 5'- gCUCUGC---ACAGGAGGCCCGAc-- -3' miRNA: 3'- aGGGACGuugUGUCCUCCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 88025 | 0.73 | 0.590559 |
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Target: 5'- gCCCcGCGGCaggaggccccgcgGCAGGAGGCCCGcggCAg -3' miRNA: 3'- aGGGaCGUUG-------------UGUCCUCCGGGUua-GU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 88070 | 0.73 | 0.550931 |
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Target: 5'- gCCCcGCG--GCAGGAGGCCCAG-CGg -3' miRNA: 3'- aGGGaCGUugUGUCCUCCGGGUUaGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 116567 | 0.74 | 0.528929 |
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Target: 5'- aUCCgUGCcccccucccccCGCGGGAGGCUCAAUCGg -3' miRNA: 3'- -AGGgACGuu---------GUGUCCUCCGGGUUAGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 87951 | 0.77 | 0.350243 |
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Target: 5'- gCCCcGCGGCaggaggccccacgGCAGGAGGCCCAGUa- -3' miRNA: 3'- aGGGaCGUUG-------------UGUCCUCCGGGUUAgu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 123766 | 1.08 | 0.003304 |
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Target: 5'- cUCCCUGCAACACAGGAGGCCCAAUCAc -3' miRNA: 3'- -AGGGACGUUGUGUCCUCCGGGUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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