Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33361 | 5' | -55.6 | NC_007605.1 | + | 123766 | 1.08 | 0.003304 |
|
Target: 5'- cUCCCUGCAACACAGGAGGCCCAAUCAc -3' miRNA: 3'- -AGGGACGUUGUGUCCUCCGGGUUAGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 125621 | 0.68 | 0.818255 |
|
Target: 5'- gCCCUuCAGCggGCGGGAGGCCagacUCAa -3' miRNA: 3'- aGGGAcGUUG--UGUCCUCCGGguu-AGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 126301 | 0.66 | 0.901754 |
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Target: 5'- uUCCCUu---CGCAGGAGGgccaCCAGUCu -3' miRNA: 3'- -AGGGAcguuGUGUCCUCCg---GGUUAGu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 132290 | 0.68 | 0.851513 |
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Target: 5'- aCCCUGCc---CAGGGGaGCCCAGcCAc -3' miRNA: 3'- aGGGACGuuguGUCCUC-CGGGUUaGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 133752 | 0.66 | 0.922078 |
|
Target: 5'- cUCCCUGCcuCACGGGaAgagcugcuggagcugGGCCCGGa-- -3' miRNA: 3'- -AGGGACGuuGUGUCC-U---------------CCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 143353 | 0.66 | 0.930678 |
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Target: 5'- gUCCCUGCcuGGCAcCAGGGuccGGCCUGGg-- -3' miRNA: 3'- -AGGGACG--UUGU-GUCCU---CCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 145430 | 0.67 | 0.874369 |
|
Target: 5'- gUCCCaaaUGUcaccacagAACACAGGGGGCUgGAUUu -3' miRNA: 3'- -AGGG---ACG--------UUGUGUCCUCCGGgUUAGu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 164115 | 0.72 | 0.62241 |
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Target: 5'- gCUCUGC---ACAGGAGGCCCGAc-- -3' miRNA: 3'- aGGGACGuugUGUCCUCCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 165110 | 0.66 | 0.925376 |
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Target: 5'- gCCUggccgGCGgccACACAGGAGGCCa----- -3' miRNA: 3'- aGGGa----CGU---UGUGUCCUCCGGguuagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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