Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33361 | 5' | -55.6 | NC_007605.1 | + | 164115 | 0.72 | 0.62241 |
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Target: 5'- gCUCUGC---ACAGGAGGCCCGAc-- -3' miRNA: 3'- aGGGACGuugUGUCCUCCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 30121 | 0.71 | 0.653301 |
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Target: 5'- cCUCUGgccCGAUACGGGGGGCCCGGg-- -3' miRNA: 3'- aGGGAC---GUUGUGUCCUCCGGGUUagu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 121260 | 0.71 | 0.684038 |
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Target: 5'- gCCCUGCAGCuuucuGCAGGAGGCauuCCccgCAc -3' miRNA: 3'- aGGGACGUUG-----UGUCCUCCG---GGuuaGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 58704 | 0.7 | 0.724369 |
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Target: 5'- aUCCUGCAguacguggggGCAcCAGG-GGCCCAGUaCAc -3' miRNA: 3'- aGGGACGU----------UGU-GUCCuCCGGGUUA-GU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 112166 | 0.7 | 0.743111 |
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Target: 5'- aCgCUGCGGCACGGGgcaaaagGGGCCCuauUCu -3' miRNA: 3'- aGgGACGUUGUGUCC-------UCCGGGuu-AGu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 58881 | 0.7 | 0.744089 |
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Target: 5'- gUCCUGCAcggcaguuACaACGGGGuGGCCCAGUUc -3' miRNA: 3'- aGGGACGU--------UG-UGUCCU-CCGGGUUAGu -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 98577 | 0.7 | 0.753805 |
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Target: 5'- ----aGCAACACccccuGGGAGGCCCAcgCGu -3' miRNA: 3'- agggaCGUUGUG-----UCCUCCGGGUuaGU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 68014 | 0.69 | 0.782263 |
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Target: 5'- cUCCUUGCccagGACACAGGuGcGCCCGuaggCAa -3' miRNA: 3'- -AGGGACG----UUGUGUCCuC-CGGGUua--GU- -5' |
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| 33361 | 5' | -55.6 | NC_007605.1 | + | 123766 | 1.08 | 0.003304 |
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Target: 5'- cUCCCUGCAACACAGGAGGCCCAAUCAc -3' miRNA: 3'- -AGGGACGUUGUGUCCUCCGGGUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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